SpectroLight 600/610

Workflow Monitoring

Workflow monitoring fills a common gap in information about the state of a sample after chromatographic purification. Although the protein is usually highly pure after chromatography, purity alone is often only one critical sample parameter among others that must be adjusted for subsequent structural characterisation. Another critical parameter is the concentration, which is often low after a chromatography step. As a result, the concentration of a sample usually has to be increased afterwards. Sample storage is also often unavoidable. Each of these sample treatments is relevant in terms of forcing a protein into aggregation, as freezing or overconcentration are known to potentially have such effects. A very powerful way of maintaining sample quality after chromatography steps is to monitor them frequently using in situ DLS. On the one hand, it is capable of revealing the dispersity of a sample even in incredibly small aliquots. It is also a simple method that can be performed many times a day.

 

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