Spectro Light 600
ONE FOR ALL

Spectro Light 600/610

is a powerful desktop device capable to measure particle sizes fully automated via dynamic light scattering (DLS) in unmatched small sample volumes (0.08 – 1 µl). Standard 96 well format plates, provided by many laboratory supplying companies, are used as sample carriers. These features make SpectroLight 600 ideal for many laboratory applications just as sample quality or stability analysis, buffer screening and many more. Besides DLS, Spectrolight provides a fully-fledged built-in laboratory microscope for the visual inspection of samples, which is well-proven to be of great help when it comes to registering colloidal or solid impurities. Furthermore, the imaging capabilities extend the application spectrum of SpectroLight 600. It can be used as a fully automated imaging system for rapid crystal identification with optional UV imaging.

Make your sample fit for modern analysis techniques

Once a sample has been identified to be monodisperse, it is already qualified for nearly all subsequent structural determination methods, since a uniform population of proteins in solution is essential, for highly reliable data.

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One for All

SpectroLight 600 provides unmatched versatility in its application spectrum. It is not only the most sample efficient high-throughput microwell plate-based dynamic light scattering instrument on the market, but it is also a fully-fledged imaging system. Optional configurations allow for meeting customer-specific requirements.

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Buffer screening

In situ DLS is a rapid and reliable method for scoring protein responses to a multitude of buffer conditions based on the particle size.

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Unmatched small Sample Volume

A key feature of sample efficiency is the capability to perform measurements on volumes as small as possible. With standard volumes between 80 to 500 nl/well, in micro-well plates, SpectroLight 600 enables the measurement of DLS on unmatched small sample volumes.

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Non-Invasiveness

A non-invasive and direct detection method that registers moving particles in solution without changing them, that is close to the optimum detection priciple generally conceivable. And exactly this is what in situ DLS is about.

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Particle Size Determination

Particle sizes can be determined in droplets, which is called "in situ" by applying a laser beam directly into a sample drop located in a well of a plate. Particles can be measured in a range of 1 nm up to a few micrometres. That closes the gap, between the vis-microscope observable and the molecular world. Based on hydrodynamics, particle size information can be obtained with +/- 2% accuracy and determined even when they coexist in a mixture of several populations.

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Workflow Monitoring

Quick data acquisition and a direct output on small aliquots are enabled by situ DLS and these are key features of an "all over monitoring" approach. Keeping a sample monodisperse throughout all steps indicates a "feeling good" situation for the protein.

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REFERENCES

James Birch , Danny Axford , James Foadi , Arne Meyer , Annette Eckhardt , Yvonne Thielmann , Isabel Moraes

The fine art of integral membrane protein crystallisation, Methods 2018 Sep 1;147:150-162. doi: 10.1016/j.ymeth.2018.05.014. Epub 2018

Karsten Dierks, Arne Meyer, Howard Einspahr and Christian Betzel

Dynamic Light Scattering in Protein Crystallization Droplets: Adaptations for Analysis and Optimization of Crystallization Processes, Cryst. Growth Des., 2008, 8 (5), pp 1628–1634

Dominik Oberthuer, Emilio Melero-García, Karsten Dierks, Arne Meyer, Christian Betzel, Alfonso Garcia-Caballero and Jose A. Gavira

Monitoring and Scoring Counter-Diffusion Protein Crystallization Experiments in Capillaries by in situ Dynamic Light Scattering,
PLoS ONE 7(6): e33545. doi:10.1371/journal.pone.0033545

Arne Meyer, Karsten Dierks, Rana Hussein, Karl Brillet, Hevila Brognaro, and Christian Betzel

Systematic analysis of protein-detergent complexes applying dynamic light scattering to optimize solutions for crystallization trials,
Acta Crystallogr F Struct Biol Commun. 2015 Jan 1; 71(Pt 1): 75–81