a true milestone in crystallization of biological macromolecules
Adjusting crystallization conditions based on real time monitoring of molecular interactions.
Cryo protection often leads to an osmotic shock of a crystal since mother liquor is significantly changed with osmotic active substances.
High degree of cross-linking
Crosslinking crystals to a high degree can be challenging as the initial concentration of such reactive agents must be carefully considered. In addition, side reactions can occur which negatively affect the degree of crosslinking. Picotitration of fresh crosslinker provides shock free long term crosslinking.
A common and non-destructive approach to producing nanocrystals is to avoid interphases by picolitre titration. The resulting crystals can be as small as crystals just at the critical radius.
Applications (PDF files)
– Classical Crystallization (~1.0 mm)
– Micro-Crystallization (~25 µm)
– Nano-Crystallization (~ 150 nm)
– Minimum Osmolarity Cryro-Protection
Karsten Dierks, Arne Meyer, Howard Einspahr and Christian Betzel
Dynamic Light Scattering in Protein Crystallization Droplets: Adaptations for Analysis and Optimization of Crystallization Processes, Cryst. Growth Des., 2008, 8 (5), pp 1628–1634
Dominik Oberthuer, Emilio Melero-García, Karsten Dierks, Arne Meyer, Christian Betzel, Alfonso Garcia-Caballero and Jose A. Gavira
Monitoring and Scoring Counter-Diffusion Protein Crystallization Experiments in Capillaries by in situ Dynamic Light Scattering,
PLoS ONE 7(6): e33545. doi:10.1371/journal.pone.0033545
Arne Meyer, Karsten Dierks, Rana Hussein, Karl Brillet, Hevila Brognaro, and Christian Betzel
Systematic analysis of protein-detergent complexes applying dynamic light scattering to optimize solutions for crystallization trials,
Acta Crystallogr F Struct Biol Commun. 2015 Jan 1; 71(Pt 1): 75–81