Interactive Crystallization

Interactive Crystallization

a true milestone in crystallization of biological macromolecules

Interactive Crystallization

Adjusting crystallization conditions based on real time monitoring of molecular interactions.

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Cryo protection

Cryo protection often leads to an osmotic shock of a crystal since mother liquor is significantly changed with osmotic active substances.

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High degree of cross-linking

Cross-linking of crystals to a high degree can be challenging since the initial concentration of such reactive agens has to be carefully examined. In addition, side reactions may occur negatively affecting the degree of cross-linking. Picotitration of fresh crosslinker provides a shock free long term cross-linking.

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Nanocrystal Production

A commonly applicable and destruction free approach for producing nano crystals is to avoid interphases by picoliter titration. Resulting crystals can be as small as crystals can be just at the critical radius.

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REFERENCES

Karsten Dierks, Arne Meyer, Howard Einspahr and Christian Betzel

Dynamic Light Scattering in Protein Crystallization Droplets: Adaptations for Analysis and Optimization of Crystallization Processes, Cryst. Growth Des., 2008, 8 (5), pp 1628–1634

Dominik Oberthuer, Emilio Melero-García, Karsten Dierks, Arne Meyer, Christian Betzel, Alfonso Garcia-Caballero and Jose A. Gavira

Monitoring and Scoring Counter-Diffusion Protein Crystallization Experiments in Capillaries by in situ Dynamic Light Scattering,
PLoS ONE 7(6): e33545. doi:10.1371/journal.pone.0033545

Arne Meyer, Karsten Dierks, Rana Hussein, Karl Brillet, Hevila Brognaro, and Christian Betzel

Systematic analysis of protein-detergent complexes applying dynamic light scattering to optimize solutions for crystallization trials,
Acta Crystallogr F Struct Biol Commun. 2015 Jan 1; 71(Pt 1): 75–81